| Primary Identifier | MGI:6155691 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Smchd1 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting construct introduced loxP sites surrounding exon 4 of mouse Smchd1 gene and a neomycin cassette flanked by Frt sites by recombineering. The neomycin cassette was removed by crossing with the FLPe knockin mouse strain. Cre-mediated recombination of Smchd1tm1.1Maar removed exons 4. Western blot analysis onfirmed the absence of wild-type protein in homozygous embryos at E12.5. |
