| Primary Identifier | MGI:6161212 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inducible, Inserted expressed sequence, Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | ES cell line = a mutant A17 line (A2Lox.cre) that carries a doxycycline-inducible Cre recombinase ORF flanked by loxP and lox2272 sites, upstream of a non-functional neomycin resistance (neo) gene. |
| molecularNote | The targeting vector was designed with a human MIR125B1 cDNA inserted between 2 splicing sites in the 3' untranslated region of an EGFP sequence and followed by a polyA signal. The targeting vector was then used to replace the Cre ORF in a mutant embryonic stem cell line (see note) and placed under the control of the tet response promoter (TRE). The construct was inserted into a cassette exchange locus upstream of the Hprt gene using Inducible Cassette Exchange (ICE) recombination. |
