| Primary Identifier | MGI:6160287 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Rnf214 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences CATCCAAAGAGGTTACACAT, CTTTCTACCCAACAATTTGT, CTACTACTAAAATGGAATTG and GGAGAAGGCCTATAGCATCT, which resulted in a 992 bp deletion beginning at Chromosome 9 position 45,899,524 bp and ending after 45,900,515 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001272251 (exon 3) and 481 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 35 and early truncation 10 amino acids later. |
