| First Author | Zhang Y | Year | 2018 |
| Journal | PLoS One | Volume | 13 |
| Issue | 2 | Pages | e0193184 |
| PubMed ID | 29474443 | Mgi Jnum | J:259077 |
| Mgi Id | MGI:6140860 | Doi | 10.1371/journal.pone.0193184 |
| Citation | Zhang Y, et al. (2018) Amyloid formation reduces protein kinase B phosphorylation in primary islet beta-cells which is improved by blocking IL-1beta signaling. PLoS One 13(2):e0193184 |
| abstractText | Amyloid formation in the pancreatic islets due to aggregation of human islet amyloid polypeptide (hIAPP) contributes to reduced beta-cell mass and function in type 2 diabetes (T2D) and islet transplantation. Protein kinase B (PKB) signaling plays a key role in the regulation of beta-cell survival, function and proliferation. In this study, we used human and hIAPP-expressing transgenic mouse islets in culture as two ex vivo models of human islet amyloid formation to: 1. Investigate the effects of amyloid formation on PKB phosphorylation in primary islet beta-cells; 2. Test if inhibition of amyloid formation and/or interleukin-1beta (IL-1beta) signaling in islets can restore the changes in beta-cell phospho-PKB levels mediated by amyloid formation. Human and hIAPP-expressing mouse islets were cultured in elevated glucose with an amyloid inhibitor (Congo red) or embedded within collagen matrix to prevent amyloid formation. To block the IL-1beta signaling, human islets were treated with an IL-1 receptor antagonist (anakinra) or a glucagon-like peptide-1 agonist (exenatide). beta-cell phospho-PKB levels, proliferation, apoptosis, islet IL-1beta levels and amyloid formation were assessed. Amyloid formation in both cultured human and hIAPP-expressing mouse islets reduced beta-cell phospho-PKB levels and increased islet IL-1beta levels, both of which were restored by prevention of amyloid formation either by the amyloid inhibitor or embedding islets in collagen matrix, resulting in improved beta-cell survival. Furthermore, inhibition of IL-1beta signaling by treatment with anakinra or exenatide increased beta-cell phospho-PKB levels, enhanced proliferation and reduced apoptosis in amyloid forming human islets during 7-day culture. These data suggest that amyloid formation leads to reduced PKB phosphorylation in beta-cells which is associated with elevated islet IL-1beta levels. Inhibitors of amyloid or amyloid-induced IL-1beta production may provide a new approach to restore phospho-PKB levels thereby enhance beta-cell survival and proliferation in conditions associated with islet amyloid formation such as T2D and clinical islet transplantation. |
