Primary Identifier | MGI:6161761 | Allele Type | Targeted |
Attribute String | Conditional ready, Inserted expressed sequence | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | C57BL/6 |
Is Recombinase | false | Is Wild Type | false |
description | ES Cells: BCGEN B61-6 |
molecularNote | A minigene composed of the coding sequences of mouse Cyp1a1 and rat Thy1 connected by a 2A sequence followed by the Woodchuck hepatitis virus derived regulatory element and a bovine growth hormone polyadenylation site was inserted into the locus. In addition a cassette containing an FRT-flanked neomycin-resistance gene and loxP-flanked transcriptional stop elements were inserted upstream of the minigene. This creates a conditional-ready allele that will only be expressed after cre-mediated deletion of the STOP cassette. |