| First Author | Bonod-Bidaud C | Year | 2012 |
| Journal | J Invest Dermatol | Volume | 132 |
| Issue | 7 | Pages | 1841-9 |
| PubMed ID | 22437311 | Mgi Jnum | J:189446 |
| Mgi Id | MGI:5445825 | Doi | 10.1038/jid.2012.56 |
| Citation | Bonod-Bidaud C, et al. (2012) In vivo evidence for a bridging role of a collagen V subtype at the epidermis-dermis interface. J Invest Dermatol 132(7):1841-9 |
| abstractText | Collagen V is the defective product in most cases of classical Ehlers-Danlos syndrome (EDS), a connective tissue disorder typically characterized by skin fragility and abnormal wound healing. Collagen V assembles into diverse molecular forms. The predominant alpha1(V)(2)alpha2(V) heterotrimer controls fibrillogenesis in skin and other tissues. The alpha1(V)(3) minor form is thought to occur in skin, but its function is unknown. To elucidate its role, we generated transgenic mice that overexpress the human alpha1(V)(3) homotrimer in the epidermis. The transgene-derived product is deposited as thin unstriated fibrillar material in the basement membrane zone of embryonic and perinatal epidermis and hair follicles. Accumulation of alpha1(V)(3)-containing fibrils leads to ultrastructural modifications at the epidermis-dermis interface and provokes changes in biomechanical properties, although not statistically significant. Using superparamagnetic immunobeads to isolate authentic suprastructures and protein-binding assays, we demonstrate that the homotrimer is part of a protein network containing collagen IV, laminin-111, and the dermal collagen VI. Our data show that the homotrimer serves as a bridging molecule that contributes to the stabilization of the epidermal-dermal interface. This finding strongly suggests that collagen V may be expressed in skin as different subtypes with important but distinct roles in matrix organization and stability. |
