| Primary Identifier | MGI:6200320 | Allele Type | Targeted |
| Attribute String | No functional change, Recombinase, Reporter | Gene | Nphs2 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting construct designed to insert a codon improved Cre Recombinase gene (icre) and a membrane-targeted tandem dimer tdTomato (mTomato) under the control of the endogenous Nphs2 promoter using viral T2A-peptides was generated using bacterial artificial chromosome clones from the C57BL/6J RPCIB-731 BAC library. The T2A.icre.T2A.mTomato cassette was inserted between the last codon of exon 8 of Nphs2 and the stop codon. A frt-flanked Puromycin resistance cassette was placed in intron between exon 7 and exon 8. The constitutive knock-in allele was generated after Puromycin resistance cassette was removed by in vivo Flp-mediated removal of the selection marker. |
