| Primary Identifier | MGI:6195701 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Hoxd10 |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Starting with genomic DNA from the Del(2Hoxd1-Hoxd9)63Ddu allele, a 10 bp deletion (CCGACAGGCA) was created Using sgRNA 5'-AGAGCGTTAACCTCACCGAC,-3'. This deletion affects the coding region, introducing a frameshift and premature stop codon that replaces the last 34 amino-acids of the encoded peptide with 10 new amino-acids. This truncated peptide has a disrupted homeodomain with the third alpha-helix eliminated. |
