| Primary Identifier | MGI:6201351 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Hcrt |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | Bacterial artificial chromosome clones containing the preprohypocretin (Hcrt) gene were used to target a cassette containing the internal ribosomal entry sequence (IRES) linked to Cre recombinase and a Frt-flanked neomycin resistance gene immediately downstream of the exon 2 stop codon. The target construct was electroporated into C57BL/6 embryonic stem cells, targeted clones were injected into blastocysts, and resultantchimeras were bred for germline transmission and then crossed with Flp-deletion mice to remove the neomycin selection gene. |
