| Primary Identifier | MGI:6201444 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Slc9a9 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 94691494 of Chromosome 9 upstream of the exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, an SV40 polyA cassette, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 94692377. Exon 5 is thus flanked by loxP sites. Flp mediated recombination created a "conditional ready" (floxed) allele. Subsequent cre expression created a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
