Primary Identifier | MGI:6196022 | Allele Type | Targeted |
Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
description | This allele was produced in zygotes collected from mice carrying the Gt(ROSA)26Sortm12Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl+)-derived R1 ES cells. |
molecularNote | The transgenic construct contains from 5' to 3' an attB site, a chicken beta-actin promoter (with cytomegalovirus immediate early enhancer), a Lox3172 site, mCherry reporter with a polyA tail, a 7.2 kb spacer, a loxP site, Lox3172 site, membrane bound enhanced yellow fluorescent protein (meYFP), woodchuck hepatitis virus posttranscriptional regulatory element and polyadenylation signal (WPRE-pA),a loxP site and an attB site and is integrated into the Gt(ROSA)26Sor locus using PhiC31 integrase RNA. |