Primary Identifier | MGI:6267327 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Ssu72 |
Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
Is Recombinase | false | Is Wild Type | false |
Project Collection | IMPC |
molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GCCCTAACATGGGAAGCACA and ATACAGGTCAGAGGATGTGG, which resulted in a 439 bp deletion beginning at Chromosome 4 position 155,731,135 bp and ending after 155,731,573 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000184815 (exon 3) and 299 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 75 and early truncation 23 amino acids later. |