| Primary Identifier | MGI:6269386 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Vkorc1l1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCCATGACTGATTTGCGCAA and TTTACTATGAAGACACACTA, which resulted in a 325 bp deletion beginning at Chromosome 5 position 129,978,373 bp and ending after 129,978,697 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000410731 (exon 2) and 215 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 65 and early truncation 62 amino acids later. |
