| Primary Identifier | MGI:6270374 | Allele Type | Targeted |
| Attribute String | Inducible, Knockdown, Reporter, RMCE-ready | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A replacement vector (TRE-tGFP-miRE\Sod2_835_shRNA_1) was constructed to have an FRT site, a Tet-responsive element followed by the constitutively active, minimal human cytomegalovirus promoter, an IRES-turboGFP sequence (tGFP; a fast-maturating and short-half-life green fluorescent protein variant); Sod2_835_shRNA_1 (placed in the tGFP 3' UTR), an RBG polyA sequence and an FRT site. The Sod2_835_shRNA_1 is comprised of a 141 bp shRNA sequence that yields significant reduction of SOD2 expression at the mRNA and protein level. The vector is inserted in the Col1a1 locus. |
