| Primary Identifier | MGI:6274391 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Hesx1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTACCATTCATTTTTCAGTG and AGCCAATAGTGACAACACAA, which resulted in a 490 bp deletion beginning at Chromosome 14 position 27,001,112 bp and ending after 27,001,601 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000249582 (exon 2) and 200 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 52 and early truncation 8 amino acids later. |
