Primary Identifier | MGI:6280650 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Tie1 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The first loxP was inserted into intron 14, and the second loxP together with neomycin-resistant gene flanked by FRT sites was inserted into intron 16. The Neo-cassette was removed using FLPeR mice, leaving exons 15 and 16 floxed. The 2 exons encode part of Tie1 intracellular domain (ICD). |