| First Author | Suzuki N | Year | 2019 |
| Journal | Sci Rep | Volume | 9 |
| Issue | 1 | Pages | 19882 |
| PubMed ID | 31882770 | Mgi Jnum | J:298483 |
| Mgi Id | MGI:6480168 | Doi | 10.1038/s41598-019-56488-7 |
| Citation | Suzuki N, et al. (2019) Laminin alpha2, alpha4, and alpha5 Chains Positively Regulate Migration and Survival of Oligodendrocyte Precursor Cells. Sci Rep 9(1):19882 |
| abstractText | In the developing central nervous system (CNS), oligodendrocyte precursor cells (OPCs) migrate along blood vessels and are widely distributed in the CNS. Meanwhile, OPCs require survival factors from the extracellular microenvironment. In other tissues, laminins, heterotrimetric (alphabetagamma) extracellular matrix proteins, promote cell migration and survival. However, the expression pattern and functions of laminins in OPC development remain poorly understood. In the present study, we first investigated the expression of laminin alpha chains, which bind to cell surface receptors such as integrins, in the postnatal murine brain. We found that laminin alpha1, alpha2, alpha4, and alpha5 chains were expressed around blood vessels and OPCs attached the laminin alpha chain-positive vessels. We then evaluated the effect of these laminins on OPCs activity using recombinant laminin E8s (LME8s) that are minimally active fragments of the laminin isoforms. OPCs attached on LM211E8, LM411E8, and LM511E8, containing laminin alpha2, alpha4, and alpha5 chains, respectively, through integrin beta1. Further, these three LME8s promoted migration of OPCs, and OPC survival was prolonged on either LM411E8 or LM511E8 via the activation of focal adhesion kinase. Together, our findings suggest that laminins expressed surrounding blood vessels positively regulate migration and survival of OPCs through the integrin beta1-FAK pathway. |
