| Primary Identifier | MGI:6272824 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Reporter | Gene | Tg(ACTA1-ATP2A1*/DsRed/GFP)5Wheel |
| Strain of Origin | FVB/N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgenic construct is designed with a human skeletal actin (ACTA1 or HSA) promoter followed by a human ATP2A1 exon 22 minigene with a single base deletion to reduce the number of base pairs from 42 to 41. The deletion allows transcription to bypass a stop codon and shift the reading frame. The ATP2A1 minigene is followed by sequence from a DsRed reporter fused to a GFP reporter. If alternative splicing includes exon 22 then the DsRed sequence is in-frame; if exon 22 is skipped then the reading frame shifts to GFP. The bichromatic shift occurs because DsRed has two open reading frames. |
