| Primary Identifier | MGI:6281414 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Camk2a*T286D)P15Mmay |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Genomic sequence starting 8.5 kb upstream of the translation initiation site and containing some 5' exons, was fused to a cDNA containing the remaining 3' sequence (including 3' UTR). The cDNA had been mutagenized to change threonine codon 286 (ACC) to an aspartic acid codon (GAC) (p.286D), which creates a phosphomimic in the peptide. The mutant peptide is 20- to 30-fold more active as a kinase in the absence of Ca(2+) than the wild-type form. Three mouse lines successfully transmitted the allele, and lines P3 and P15 were studied further. Line P15 has 1 copy of the transgene. Northern blot and RT-PCR experiments showed expression of the transgene. |
