| Primary Identifier | MGI:6286225 | Allele Type | Targeted |
| Attribute String | Epitope tag, Humanized sequence | Gene | Nkx2-5 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 2 was replaced with one containing a CGC to TGC non-synonymous substitution resulting in an arginine to cysteine change at amino acid 141 (R141C), an frt-loxP-neo-frt-loxP cassette was inserted upstream of exon 2, and a flag tag was inserted at the ATG of exon 1. Flp-mediated recombination removed the neo selection cassette. This mutation is identified in patients with congenital heart defects. |
