| Primary Identifier | MGI:6281343 | Allele Type | Targeted |
| Gene | Pgf | Transmission | Germline |
| Strain of Origin | 129 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Two point mutations that result in an aspartate to alanine substitution at amino acid 67 and a glutamate to alanine substitution at amino acid 68 were introduced in exon 3 and a loxP flanked PGK-neomycin cassette was inserted upstream of exon 3. Cre-mediated recombination removed the neomycin cassette. These two mutations generate the PGF-DE variant seen in humans that results in the loss of binding and activation of FLT1 (VEGFR-1) but still maintains the ability to heterodimerize with VEGFA. |
