Primary Identifier | MGI:6314348 | Allele Type | Gene trapped |
Attribute String | Null/knockout | Gene | Ranbp1 |
Transmission | Germline | Strain of Origin | 129S4/SvJaeSor |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The insertion site was mapped to the 98th base of exon 2 of Ranbp1. The beta-geo reporter is almost undetectable due to the atypical insertion of the reporter into the exon rather than intron. The hygromycin cassette was excised via Flp-mediated recombination. Western blotting and immunofluorescence staining confirmed absence of protein and no 44 amino acid residual protein is detected. |