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Allele : Gt(ROSA)26Sor<em1.1(CAG-RFP*)Jrt> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, Janet Rossant

Primary Identifier  MGI:6383974 Allele Type  Endonuclease-mediated
Attribute String  Reporter Gene  Gt(ROSA)26Sor
Strain of Origin  CD-1 Is Recombinase  false
Is Wild Type  false
molecularNote  CRISPR/cas9 endonuclease-mediated genome editing was used to introduce a CAG-H2B-miRFP703 (nuclear localized) vector into the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette. The STOP cassette was removed by an in vitro cre reaction on the plasmid. miRFP703 is derived from miRFP670, which is itself derived from RpBphP1, a bacterial phytochrome photoreceptor, Rhodopseudomonas palustris. miRFP703 includes the following mutations: D16S/L17H/C20S/R22H/P43H/I47V/D106C/P127S/A155V/E179L/R184C/D201T/I202V/R208Q/E211V/S222T/I253C/Y257F/N260D/V268A/A282V/H292R/E300K/L301R/A305R/S20C/T201L/C253I.
  • mutations:
  • Insertion
  • synonyms:
  • miRFP703,
  • R26-CAG-H2B-miRFP703,
  • R26-CAG-H2B-miRFP703,
  • miRFP703
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

0 Driven By

3 Publication categories

Trail: Allele