| Primary Identifier | MGI:6383980 | Allele Type | Endonuclease-mediated |
| Attribute String | Reporter | Gene | Sox2 |
| Strain of Origin | 129 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/cas9 endonuclease-mediated genome editing is used to target the super enhancer (SE) sequences ~100 kb distal of the SRY (sex determining region Y)-box 2 locus (Sox2) on chromosome 3, as well as Snrpn::tdTomato::WPRE::BHGpA::loxP-PGK-Puro-loxP sequences (encoding the minimal Snrpn promoter, the tdTomato fluorescent protein, a WPRE sequence, a BGH polyadenylation sequence and a loxP-flanked PGK-Puro selection cassette). Junction PCR-SNP analyses identified ES cells with the S2T sequences (Snrpn::tdTomato::WPRE::BHGpA) inserted into the Sox2 SE on the 129 chromosome. Those ES cells were transfected with a Cre recombinase-expressing plasmid to remove the PGK-Puro, |
