| Primary Identifier | MGI:6295411 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Ncmap |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences AGGTTCAAACCCAAACCATG and TCAGACATCTATGAGTCCCC, which resulted in a 289 bp deletion beginning at Chromosome 4 position 135,371,731 bp and ending after 135,372,019 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001225043 (exon 4) and 204 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 25 and early truncation 2 amino acids later. |
