| Primary Identifier | MGI:6283570 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Mamdc2 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CATGTGTTCTATGCTATATG and CTTGCATTGCATCATCCCAA, which resulted in a 446 bp deletion beginning at Chromosome 19 position 23,378,605 bp and ending after 23,379,050 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000349008 (exon 3) and 174 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 49 and early truncation 13 amino acids later. There is a single bp (T) insertion at the deletion site. |
