| Primary Identifier | MGI:6281947 | Allele Type | Targeted |
| Attribute String | Inducible, Inserted expressed sequence, Reporter | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm2(tetO-Pou5f1)Jae. |
| molecularNote | The targeting vector contains sequence encoding the mouse Gfi1 open reading frame, followed by an IRES-Venus (YFP variant) under the control of the tetO (tetracycline responsive element). The Gfi1-IRES-Venus vector is inserted into the 3' UTR of the Col1a1 locus using a FLP recombinase expression vector (pCAGs-FLPe-Puro), which also repairs a defective hygromycin resistance gene. |
