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Allele : Col1a1<tm2(Gt(ROSA)26Sor-rtTA,tetO-GFP/RNAi:rluc)Hail> collagen, type I, alpha 1; targeted mutation 2, Stephan Hailfinger
Primary Identifier  MGI:6295871 Allele Type  Targeted
Attribute String  Inducible, Reporter, Transactivator Gene  Col1a1
Transmission  Germline Strain of Origin  Not Specified
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
molecularNote  The transgene construct was inserted using recombinase-mediated cassette exchange (RMCE). The transgene construct contains the following elements: the Gt(ROSA)26Sor promoter driving the expression of the reverse tetracycline-controlled transactivator, and the tet operon tetracycline responsive element driving the expression of turboGFP green fluorescent protein reporter. The GFP gene is fused with sequence for a miR30-based shRNA targeting Renilla reniformis luciferase. Only upon supplying mice carrying this allele with doxycycline (or tetracycline) will the GFP/shRNA hybrid gene be expressed.
  • mutations:
  • Insertion
  • synonyms:
  • ROSA26-rtTA TRE-turboGFP-shRenilla,
  • ROSA26-rtTA TRE-turboGFP-shRenilla
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Trail: Allele

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0 Mutation Involves

Phenotype

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