Primary Identifier | MGI:6284311 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mir574 |
Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | false | Is Wild Type | false |
Project Collection | Sanger miRNA knockouts |
molecularNote | A targeting vector containing (from 5' to 3') a loxP-F3-puromycin Δtk-loxP-FRT was used to disrupt Mir574 on chromosome 5. Mir574 is located in intron 1 of the host gene Fam114a1. Cre-mediated recombination removed the floxed purumycin cassette. |