| Primary Identifier | MGI:6285518 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Kirrel2 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 30154397 of Chromosome 7 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 30155529. Exons 3 and 4 are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm2a allele to remove the neo selection cassette and loxP-flanked exons 3 and 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. In situ hybridization and immunohistochemical analyses confirmed the absence of transcript and protein expression in the vomeronasal organ (VNO) and on vomeronasal sensory neuron (VSN) axons of homozygous mutant mice, respectively. |
