| Primary Identifier | MGI:6295979 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Palb2 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This allele was derived from the Palb2tm1.1Dli allele, which has a loxP site in intron 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP iste in intron 3. A mutation was introduced into part of exon 2 coding for the CC (coiled-coil) domain using site-directed mutagenesis. The TTGAAAAAG>GCAGCAGCT nucleotide substitution changes peptide sequence LeuLysLys to AlaAlaAla in the encoded protein (p.(L24_K26delinsAAA)). This amino-acid trio represents residues 15-18 of the 33 aa CC motif and the substitution negatively affects binding to Brca1. The neo cassette was removed through flp-mediated recombination. |
