| Primary Identifier | MGI:6296741 | Allele Type | Targeted |
| Attribute String | Inducible, Null/knockout, Recombinase, Reporter | Gene | Fgf10 |
| Transmission | Germline | Strain of Origin | C57BL/6NCrl |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| description | S3 = C57BL/6NCrl |
| molecularNote | An EGFP gene and inducible cre/ERT2 fusion were inserted into the 3' UTR immediately downstream of the stop codon of the exon 3. The self-cleavage LF2A and T2A sequences will lead to 3 distinct proteins. Flp-mediated recombination removed the selection cassette. The homozygous embryos suffered from lung and limb agenesis and the analysis of Fgf10 expression by qPCR indicated a drastic reduction in Fgf10 expression. It is a loss-of-function allele. |
