| Primary Identifier | MGI:6314212 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Trmt2b |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCCGTGACTCTAGTGCCATT and CTACTATTCGTAAATAGCCC, which resulted in a 199 bp deletion beginning at Chromosome X position 134,266,210 bp and ending after 134,266,408 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001222478 (exon 3) and 143 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 73 and early truncation 27 amino acids later. |
