| Primary Identifier | MGI:6304310 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Zfp512b |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ACTGGCACAGGATATATGGA and ATATCACCGACCCCCAAGGG, which resulted in a 295 bp deletion beginning at Chromosome 2 position 181,590,391 bp and ending after 181,590,685 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000170907 (exon 3) and 152 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 41 and early truncation 9 amino acids later. There is an 11 bp insertion (CCAGCCCTCAC) at the deletion site. |
