| Primary Identifier | MGI:6304317 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Agrp |
| Strain of Origin | (C57BL/6J x DBA/2J)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The gene was targeted using CRISPR/Cas9 technology. A mixture of four sgRNAs, two targeted upstream and two targeted downstream of the coding region of the gene, plus Cas9 mRNA was injected into (B6 x D2)F1 zygote pronuclei. Of the ten candidate mouse lines, line 5 was used for further experiments. The allele in these mice has a 912 bp deletion in the gene. |
