| Primary Identifier | MGI:6381415 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Lysmd1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GTTGAAAACAGTGTACTTGG and AGAAGTAACCAAAGTACCCG, which resulted in a 614 bp deletion beginning at Chromosome 3 position 95,137,456 bp and ending after 95,138,069 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000422932 (exon 2) and 252 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 60 and early truncation 2 amino acids later. |