| Primary Identifier | MGI:6323625 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(Nes-KO)70Okn |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| description | Kusabira-Orange cDNA from Molecular Biological Laboratories (MBL; Nagoya, Japan) |
| molecularNote | The transgene contains the following components: the rat nestin promoter, sequence coding for Kusabira-Orange fluorescent protein with d4-type PEST sequence at the C-terminus, rat nestin 2nd intronic enhancer, and poly(A) signal sequences. The PEST sequence was added to increase temporal resolution (reduce the protein life-span). Four mouse lines were established and line 70 was found to represent nestin expression the most accurately and with the strongest signal. |
