| Primary Identifier | MGI:6381444 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Ppa2 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ATAAAGAGCAGCGCGCGCGG and ACTGCCCCATTCTAGCTCCT, which resulted in a 223 bp deletion beginning at Chromosome 3 position 133,320,903 bp and ending after 133,321,125 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001248779 (exon 2) and 158 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 47 and early truncation 2 amino acids later. |
