| Primary Identifier | MGI:6324242 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Scml4 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ACGTGTGTAGGAACACAGAG and TCTGCCAAAGGACTTCTAGG, which resulted in a 525 bp deletion beginning at Chromosome 10 position 42,923,773 bp and ending after 42,924,297 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001277490 (exon 4) and 395 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 52 and early truncation 4 amino acids later. |
