| First Author | Rai Y | Year | 2021 |
| Journal | Neuroscience | Volume | 462 |
| Pages | 36-43 | PubMed ID | 32360594 |
| Mgi Jnum | J:339591 | Mgi Id | MGI:6710032 |
| Doi | 10.1016/j.neuroscience.2020.04.035 | Citation | Rai Y, et al. (2021) Phospholipase C beta3 is Required for Climbing Fiber Synapse Elimination in Aldolase C-positive Compartments of the Developing Mouse Cerebellum. Neuroscience 462:36-43 |
| abstractText | In the cerebellum of neonatal mice, multiple climbing fibers (CFs) form excitatory synapses on each Purkinje cell (PC). Only one CF is strengthened in each PC from postnatal day 3 (P3) to P7, whereas the other weaker CFs are eliminated progressively from approximately P7 to approximately P11 (early phase of CF elimination) and from approximately P12 to approximately P17 (late phase of CF elimination). Type 1 metabotropic glutamate receptor (mGluR1) triggers a canonical pathway in PCs for the late phase of CF elimination. Among downstream signaling molecules of mGluR1, phospholipase C beta3 (PLCbeta3) and beta4 (PLCbeta4) are expressed complementarily in PCs of aldolase C (Aldoc)-positive (+) and Aldoc-negative (-) cerebellar compartments, respectively. PLCbeta4 is reported to mediate the late phase of CF elimination in the anterior half of the cerebellar vermis which corresponds to the Aldoc (-) region. However, roles of PLCbeta3 and Aldoc in CF synapse elimination are unknown. Here, we investigated CF innervation of PCs in Aldoc-tdTomato knock-in mice that underwent lentivirus-mediated knockdown (KD) of PLCbeta3 in PCs during postnatal development. By recording CF-mediated excitatory postsynaptic currents from PCs and immunostaining CF synaptic terminals, we found that significantly higher percentage of PCs with PLCbeta3-KD remained multiply innervated by CFs in Aldoc (+) compartments after P12, which was accompanied by impaired elimination of somatic CF synapses and reduced dendritic CF translocation. In contrast, deletion of Aldoc had no effect on CF synapse elimination. These results suggest that PLCbeta3 is required for the late phase of CF elimination in Aldoc (+) PCs. |
