| Primary Identifier | MGI:6324704 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Rag2 |
| Strain of Origin | C57BL/6JBomTac | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A p.Arg229Gln mutation was created in the coding region by micro-injecting zygotes with Cas9 mRNA, Scr7, two sgRNAs, and an ssODN (single-stranded oligodeoxynucleotide) containing the desired GC>AG substitution plus three silent substitutions to prevent Cas9 re-cutting. The R229Q mutation is found in some severe combined immunodeficiency (SCID) and Omenn syndrome (OS) patients. |
