Primary Identifier | MGI:6356297 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Rcn3 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exons 1 and 2 were replaced with a neomycin selection cassette. Quantitative RT-PCR analysis revealed a significant reduction of mRNA levels in E18.5 homozygous mutant lungs. Western blot analysis and immunohistochemistry confirmed the absence of protein expression in E18.5 mutant lungs. |