Primary Identifier | MGI:6356365 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Slc22a14 |
Strain of Origin | C57BL/6N | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 mutagenesis induced a deletion of exons 7-9, as confirmed by genomic DNA sequencing and genome PCR. RT-PCR and Western blot analysis revealed that expression of mRNA and its product were prominently reduced in homozygous mutant testes. |