| Primary Identifier | MGI:6357834 | Allele Type | Targeted |
| Attribute String | Null/knockout, Recombinase, Reporter | Gene | Hcrt |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A targeting construct in which the MC1 promoter-driven diphtheria toxin gene, a 5.44 kb fragment at the 5' site, codon-optimized for mammalian expression Flp recombinase, including a nuclear localization signal cDNA fused to EGFP with the T2A peptide sequence, a Pgk-1 promoter-driven neomycin phosphotransferase gene (neo) flanked by two Dre recognition target (rox) sites was used for homologous recombination in ES cells to insert EGFP-2A-Flp just behind the translational initiation site of the hypocretin gene (Hcrt) in-frame. The neo cassette was removed in the ES cells by Dre recombination. This allele is a null allele confirmed by immunohistochemical studies. |
