| Primary Identifier | MGI:6358615 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Dlx6os1 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A STOP cassette (triple polyadenylation signal sequences) was inserted into exon 3, truncating Dlx6os1 splice variant Evf2. This generates an allele that doesn't express Evf1, but expresses truncated enhancer-containing form Evf2-5' that lacks the Evf2 3' end. The Evf2-5' transcript retains transcription across the Dlx5/Dlx6 ultra-conserved enhancer (UCE) Rr291 and retains Dlx6 antisense transcription. Expression of Dlx5 is increased. A loxP site flanked neomycin resistance gene cassette was removed by cre-mediated recombination. |
