Primary Identifier | MGI:6360044 | Allele Type | Targeted |
Attribute String | Reporter, Transactivator | Gene | Axin2 |
Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct contains a P2A, a reverse tetracycline transactivator (rtTA3), a T2A, an SGFP2 (strongly enhanced green fluorescent protein) with a 3XNLS (nuclear localization signal) followed by an FRT-flanked PGK neo cassette (in reverse orientation) inserted at the stop codon (TGA) in exon 11. Flp-mediated recombination removed the FRT-flanked neo cassette. |