| First Author | Chang B | Year | 2019 |
| Journal | MGI Direct Data Submission | Mgi Jnum | J:278680 |
| Mgi Id | MGI:6359894 | Citation | Chang B (2019) The eyeless 5 mutation (eyl5) in mice. MGI Direct Data Submission |
| abstractText | The eyeless 5 mutation arose spontaneously on C3H/HeSn-ocd/J (Stock# 1431). The eyeless 5 mutant male was crossed to a C57BL/6J female and their offspring were intercrossed to generate homozygotes in the F2 generation. This line was maintained by sibling breeding eyl5/eyl5 agouti eyeless mutants without ocd (osteochondrodystrophy) or the retinal degeneration 1 mutation, Pde6brd1. At generation F26, this line suddenly stopped breeding and an old male eyl5/eyl5 mouse was crossed to a C57BL/6J female and their offspring were intercrossed to generate homozygotes in the F2 generation. At the F2 generation sperm from 2 male homozygotes was cryopreserved. Mice homozygous for the eyeless 5 Jackson mutation have anophthalmia (Fig 1, A & B), and those with intact globes have microphthalmia (Fig 1, C & D) and white corneal opacity (Fig 1, E & F) comparing to wild-type control (Fig 1, G & H). This mutation was named eyeless 5 (eyl5) and mapped to mouse Chr 12 through a cross/backcross to CAST/EiJ (Fig. 2). |
