| Primary Identifier | MGI:6367834 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Lamc1 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site and an FRT site flanked neomycin resistance gene cassette were inserted into intron 27 and a second loxP site and a copy of exon 28 containing a mutation that changes glutamic acid codon 1605 to a glutamine codon (p.E1605Q) were inserted into intron 28. The neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with the wild-type exon 28 (the last coding exon) floxed. Upon cre-mediated recombination, the floxed wild-type exon is deleted and the mutated exon 28 is expressed. |
