| Primary Identifier | MGI:6385058 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | 129S5/SvEvBrd |
| Is Recombinase | false | Is Wild Type | false |
| description | 129P2/OlaHsd-derived mutant A17 embryonic stem (ES) cell line (also called A2Lox.cre). These cells carry a doxycycline-inducible cre recombinase ORF, flanked by loxP and lox2272 sites, upstream of a non-functional neomycin resistance (neo) gene. |
| molecularNote | The targeting vector was designed with tetO (TRE) promoter, upstream of human KMT2A/MLLT3 (MLL-AF9) cDNA followed by an IRES-deltaNGFR cassette. The KMT2A/MLLT3 translocation T(9:11) contains the AT hook and DNA methyltransferase from KMT2A (1444 amino acids) at the N terminal fused to the 192 C-term amino acids from MLLT3 (breakpoints are variable). MLL-AF9 expression is monitored by the co-expressed deltaNGFR on the cell surface. This construct was inserted into a cassette exchange locus upstream of the hypoxanthine guanine phosphoribosyl transferase (Hprt) gene on the X chromosome using Inducible Cassette Exchange (ICE) recombination. |
