| Primary Identifier | MGI:6368033 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence, Hypomorph | Gene | Psmb11 |
| Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A G to A (c.145G>C) mutation was engineered to change glycine codon 49 (GGC) to a serine codon (AGC) (p.G49S) in a genomic sequence fragment in the targeting vector, which also contains A loxP site flanked neomycin resistance gene cassette inserted into an intron. The targeting vector was injected into ES cells together with two gRNAs and CRISPR/Cas9 nickase mRNA encoding plasmid. The neo cassette was removed through subsequent cre-mediated recombination. The mutation mimics a polymorphism found in the human population. |
